AtpH antibody

Catalog No. ABIN249344

The Rabbit Polyclonal anti- antibody has been validated for WB. It is suitable to detect in samples from Arabidopsis thaliana, Nicotiana tabacum, Spinach and Thermosynechococcus elongatus.

Quick Overview for AtpH antibody (ABIN249344)

Target: AtpH

Reactivity: Arabidopsis thaliana, Nicotiana tabacum, Spinach, Thermosynechococcus elongatus

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Product Details

Specificity: The anti-AtpB antibody will detect the mitochondrial form of the F1 ATP synthasesubcomplex, as well as the chloroplastic CF1 ATP synthase and most knownbacterial F-type ATP synthases.

Cross-Reactivity (Details): Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

Predicted Reactivity: dicots including Glycine max, Pisum sativum, Vitis vinifera, monocolts indlucing Oryza sativa, Zea mays, trees: Populus alba, Pinus thunbergii, moss: Physcomitrella patens, green algae

Characteristics: Expected / apparent Molecular Weight of the Antigene: 8 kDa (for Arabidopsis thaliana)

Purification: serum

Immunogen: purified c subunit from Spinacia oleracea P69447

Application Details

Application Notes: 1: 1000 - 1: 10 000 with standard ECL (WB)

Comment:

please note that increased incubation at 95°C (20-30 min) prior to loading is recommended to break the multimeric c-mer structure, detection of partial ring structures (e.g. 5 or 6 subunits) may occur

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 μL of sterile water

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Target Details

Target: AtpH

Background: AGI Code: ATCG00140
F-type ATPase (ATP synthase) is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. Multiple copies of the c subunit build up the ring structure (in spinach a 14-mer of ~112 kDa) of the membrane bound Fo-part of the enzyme.

Molecular Weight: 8 kDa (for Arabidopsis thaliana)

UniProt: P69447